Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 19;12:6768. doi: 10.1038/s41467-021-26881-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A UMAP plot showing a selected population of cell NKX2-1⁺. Expression of markers that distinguish hypothalamic and telencephalic populations (NKX2-1, SIX6 and FOXG1). B Immunostaining shows the spatial segregation of ventral population arising from a light-induced SHH source at day 14. (Green NG, Magenta NKX2.1, Cyan SIX6, Red FOXG1, Gray DAPI), Scale bar = 100 μm. C Cumulative fluorescence intensity analysis (line profile) over the x-axis. x-axis displays the linear distance in μm. y-axis shows the cumulative fluorescent intensity profile in arbitrary units for each channel. Line profile shows the average (line) and SD (area) for each channel. The line profile is color-coded as the immunofluorescent channels, NG-Green, NKX2-1-Magenta, SIX6-Cyan, FOXG1-Red at day 14 quantification (n = 3 biologically independent samples). D Immunostaining shows OTP positive cells induced in proximity of the NG-T2A-SHH organizer but not in the NG-CNTRL. Light induced SHH drive the self-organization of both neural progenitors and neurons (Green NG, Magenta OTP, Gray DAPI), Scale bar = 100 μm.