Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 19;11:22593. doi: 10.1038/s41598-021-02060-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Time course of osteogenic mineralization for MPC1 and MPC2 cells. MPC1 (A) and MPC2 (B) cells were cultured in growth media (GM) or osteogenic media (OM) and stained with alizarin red to examine mineralization over the differentiation time course. (A) Mineralization of MPC1 cells cultured in OM began at day 7, which was strongly increased at Day 14, and even more robust at Day 21. (B) MPC2 cells at Day 7 in OM exhibited more robust staining compared to MPC1 at the same time. Alizarin red stain in OM continually increased with duration of differentiation. At day 28 of OM, both cell types developed similar and robust mineralization. In contrast to MPC1, MPC2 cells exhibited modest mineralization at Day 28 when maintained in the growth media. (C) Alizarin red staining was quantified using CPC extraction followed by measuring absorbance at 544 nm. Each line was tested in triplicate. Images were captured using a 10X magnification lens. (n = 3; mean + standard error). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 between GM and OM at the time point designated; ^cp < 0.001 and ^dp < 0.0001 vs day 7 within the same media type.