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. 2021 Nov 19;11:22593. doi: 10.1038/s41598-021-02060-1

Figure 5.

Figure 5

MPC cells undergo robust adipocyte differentiation. MPC cells were cultured for 4 days in growth media (GM) or adipogenic media (AM). Cells were stained with Oil Red O to examine lipid accumulation. (A) MPC1 cells grown in AM stained for Oil Red O while cultures in GM had no staining (bar = 250 μm). (B) MPC2 cells again showed no evidence of lipid formation in GM, whereas nearly all of the cells in view were stained with Oil Red O when exposed to AM. Staining was MPC2 cells was considerably greater than that of MPC1. (C) Protein lysates were assessed from each cell line and media condition for adipogenic proteins PPARγ, ADIPOQ, PLIN and FABP4 by Western blotting. (D) Lysates from four biological replicate experiments were separated by Western blotting and normalized to GAPDH. MPC1 cells only demonstrated a significant increase in FABP4 in AM conditions (gray bars) compared to GM (white bars). (E) Significant increases in PPARγ, ADIPOQ, PLIN and FABP4 under AM conditions were observed in MPC2 cells. *p < 0.05, ***p < 0.001, ****p < 0.0001, *****p < 0.00001 vs GM.