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. 2021 Nov 19;11:22593. doi: 10.1038/s41598-021-02060-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MPC1 and MPC2 cells display chondrogenic differentiation. MPC cells were grown in pellets in either growth media (GM) or chondrogenic media (CM) for 28 days then stained for Alcian Blue (AB) and Safranin-O (SO). MPC1 cells show more robust Safranin O stain under chondrogenic conditions, whereas MPC2 cells displayed strong Alcian Blue staining in chondrogenic conditions, but no clear differences in Safranin-O. (10X; bar = 250 µm).