Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 19;11:22593. doi: 10.1038/s41598-021-02060-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

MPC cells grow into cartilaginous pellets and expression chondrogenic genes. MPC cells were cultured in growth media (GM) or chondrogenic media (CM) for 28 days. (A) Under low adherence culture conditions spheroid pellets developed and pellet volumes were measured. MPC1 cells showed increased pellet volume in chondrogenic conditions (gray bars) compared to growth media (white bars). There was no change in pellet volume for MPC2 cells. (B) RNA was analyzed after 28 days of differentiation. (C) For MPC1 cells, chondrogenic conditions (CM) significantly enhanced mRNA levels of chondrocyte markers Col1a1, Col2a1, Col10, Acan, Comp, and Sox9. (D) MPC2 cells differentiated with CM showed robust upregulation of Col1a1 and Sox9. Col10 and Comp mRNA increase was more modest and there was no change in Col2a1 and Acan. n = 3; *p < 0.05, **p < 0.01, ***p < 0.001.