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. 2021 Nov 19;4:1312. doi: 10.1038/s42003-021-02844-1

Fig. 2. Effect of piR-39980 on DOX-mediated colony formation and apoptosis of HT1080 parental cells.

Fig. 2

a Colony formation assay after transfecting the cells with 20 nM piR-39980 mimic/NC_Mimic and treatment with 0.4 µM DOX. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, ***P < 0.001, Sidak’s multiple comparisons test. b Colony formation assay after transfecting the cells with 20 nM piR-39980 inhibitor/NC_inhibitor and treatment with 0.4 µM DOX. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, **P < 0.01, Sidak’s multiple comparisons test. c The apoptosis assay after transfecting the cells with 20 nM piR-39980 mimic/NC_Mimic and treatment with 0.4 µM DOX. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, ***P < 0.001, Sidak’s multiple comparisons test. d The apoptosis assay after transfecting the cells with 20 nM piR-39980 inhibitor/NC_inhibitor and treatment with 0.4 µM DOX. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, *P < 0.05, Sidak’s multiple comparisons test.