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. 2021 Nov 19;4:1312. doi: 10.1038/s42003-021-02844-1

Fig. 3. Effect of piR-39980 on DOX-accumulation in HT1080 cells.

Fig. 3

a Intracellular DOX-accumulation was evaluated by fluorescence microscopy. Scale bar: 50 μm. b Intracellular DOX-accumulation was quantified by multimode microplate reader upon transfecting the cells with 20 nM piR-39980 mimic/NC_Mimic and treating with 0.4 µM DOX. Bars, mean ± SEM; n = 5 independent experiments; ns nonsignificant, **P < 0.01, Tukey’s multiple comparisons test. c Intracellular DOX-accumulation was evaluated by fluorescence microscopy. Scale bar: 50 μm. d Intracellular DOX-accumulation was quantified by multimode microplate reader upon transfecting the cells with 20 nM piR-39980 inhibitor/ NC_Inhibitor and treating with 0.4 µM DOX. Bars, mean ± SEM; n = 5 independent experiments; ns nonsignificant, **P < 0.01, ***P < 0.001, Tukey’s multiple comparisons test. e, f DOX-accumulation was measured by flow cytometry transfected with piR-39980 mimic and inhibitor, respectively.