Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 19;4:1312. doi: 10.1038/s42003-021-02844-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Relative expression of RRM2 detected in parental HT1080 and resistant HT1080/DOX cell lines by qRT-PCR. Bars, mean ± SEM; n = 3 independent experiments; *P < 0.05, t-test. b The expression of RRM2 in HT1080/DOX cells upon transfected with 20 nM piR-39980 mimic/inhibitor as detected by qRT-PCR. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, *P < 0.05, ***P < 0.001, Tukey’s multiple comparisons test. c The expression of RRM2 in HT1080 cells upon transfected with 20 nM piR-39980 inhibitor/mimic as detected by qRT-PCR. Bars, mean ± SEM; n = 5 independent experiments; ns nonsignificant, *P < 0.05, **P < 0.01, Tukey’s multiple comparisons test. d Relative expression of CYP1A2 detected in parental HT1080 and resistant HT1080/DOX cell lines by qRT-PCR. Bars, mean ± SEM; n = 3 independent experiments; *P < 0.05, t-test. e The expression of CYP1A2 in HT1080/DOX cells upon transfected with 20 nM piR-39980 mimic/inhibitor compared to control, detected by qRT-PCR. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, *P < 0.05, **P < 0.01, Tukey’s multiple comparisons test. f The expression of CYP1A2 in HT1080 cells transfected with 20 nM piR-39980 mimic/inhibitors detected by qRT-PCR. Bars, mean ± SEM; n = 5 independent experiments; ns nonsignificant, **P < 0.01, ***P < 0.001, Tukey’s multiple comparisons test. g Luciferase reporter constructs containing wild-type/mutant piR-39980 target site in RRM2 3′-UTR were co-transfected with mimic into the HEK293 cells. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, ***P < 0.001, Sidak’s multiple comparisons test. h Luciferase reporter constructs containing wild-type/mutant piR-39980 target site in CYP1A2 3′-UTR were co-transfected with mimic into the HEK293 cells. Bars, mean ± SEM; n = 3 independent experiments; ns nonsignificant, ***P < 0.001, Sidak’s multiple comparisons test.