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Thermostability of membrane, DDM and DIBMALP preparations of β2AR
(A–C) ThermoFRET thermostability curves in (A) β2AR membranes, (B) DDM-solubilized β2AR, (C) DIBMALP-β2AR in the presence and absence of cyanopindolol (100 μM) and F-propranolol (200 nM).
(D) β2AR and A2AR TR-FRET thermostability curves obtained by measuring reduction in fluorescent F-propranolol (200 nM) and F-XAC (200 nM) binding. All curves show normalized combined data, data points show mean ± SEM, for n = 3.
