Table 1.
Ligand binding parameters of different preparations of β2AR
| Membranes |
DDM |
DIBMA |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| pIC50 | pKi | Slope | pIC50 | pKi | Slope | pIC50 | pKi | Slope | |
| Propranolol | 8.7 ± 0.13 | 9.5 ± 0.03 | 1.0 ± 0.02 | 9.0 ± 0.04 | 9.5 ± 0.03 | 1.2 ± 0.04 | 9.1 ± 0.10 | 9.6 ± 0.10 | 0.8 ± 0.30 |
| ICI 118,551 | 8.5 ± 0.10 | 9.3 ± 0.15 | 1.1 ± 0.22 | 8.5 ± 0.02 | 8.9 ± 0.10 | 1.0 ± 0.06 | 8.3 ± 0.15 | 9.1 ± 0.06 | 1.3 ± 0.23 |
| Isoprenaline | 4.7 ± 0.12 | 5.5 ± 0.20 | 1.1 ± 0.11 | 5.8 ± 0.06 | 6.3 ± 0.13 | 1.1 ± 0.09 | 5.1 ± 0.18 | 5.8 ± 0.10 | 1.1 ± 0.15 |
Values shown are mean of n = 3 individually fitted curves ±SEM, as determined by TR-FRET competition binding assays.