Table 2.
Tm (°C) ThermoFRET | Tm(°C) F-ligand | |
---|---|---|
Membrane β2AR | 62.4 ± 0.2 | – |
Membrane β2AR + F-propranolol | 61.6 ± 0.4 | 60.1 ± 0.6a |
Membrane β2AR + cyanopindolol | 63.0 ± 0.4 | – |
DDM β2AR | 35.2 ± 2.4 | – |
DDM β2AR + F-propranolol | 37.8 ± 0.4 | 36.0 ± 0.6a |
DDM β2AR + cyanopindolol | 41.9 ± 0.1 | – |
DIBMALP β2AR | – | 46.8 ± 2.1a |
DIBMALP A2A + F-XAC | – | 44.8 ± 0.7b |
Reported error values are SEM.
β2AR thermostability was measured using F-propranolol dissociation.
DIBMALP-A2A thermostability was measured using F-XAC dissociation.