REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
One ShotTM TOP10 chemically competent E.coli	Invitrogen	C404010
Chemicals, peptides, and recombinant proteins
SNAP-Lumi4-Tb	Cis bio	SSNPTBX
n-Dodecyl- β-D-maltopyranoside (DDM)	Anatrace, OH, US	D3010S
Diisobutylene Maleic acid (DIBMA)	Anatrace, OH, US	BMA1011
5% Magstrep ‘type3’ XT magnetic bead suspension	IBA Lifesciences, DE	2-4090-002
633/650 S-propranolol-red	CellAura, UK, supplied by Hello Bio, UK	Cat no. HB7817
Fluorescent XAC CA200634	CellAura, UK, supplied by Hello Bio, UK	Cat no. HB7814
ICI, 118 551 hydrochloride	Selleckchem, US	Cat no. S8114 1217094-53-5
Isoprenaline hydrochloride	Sigma	Cat no. I5627 CAS-51-30-9
(s)-(-)-Propranolol hydrochloride	Tocris, UK	Cat no. 0834 CAS 4199-10-4
Cyanopindolol hemifumerate	Tocris, UK	Cat no. 0993 CAS 69906-86-1
BODIPYTM FL L- Cystine dye	Molecular Probes, US	B20340
NuPAGETM MOPS SDS running buffer (x20)	Invitrogen	NP0001
Experimental models: Cell lines
T-RexTM-293 cells (parent cell line)	Invitrogen	Cat.no. R71007
Stable cell line T-RexTM-293 expressing TS-SNAP- β2AR	plasmid generated in this study	This study
Stable cell line T-RexTM-293 expressing TS-SNAP- A2A	plasmid generated in this study	This study
Oligonucleotides
CMV forward sequencing primer	Genewiz, UK	CGCAAATGGGCGGTAGGCGTG
BGH reverse sequencing primer	Genewiz, UK	TAGAAGGCACAGTCGAGG
Recombinant DNA
TS-SNAP- β2AR in pcDNA4/TO	This study	This study, SI
TS-SNAP- A2A in pcDNA4/TO	This study	This study, SI
Software and algorithms
GraphPad Prism 8.0.0	GraphPad software, CA, US	www.graphpad.com
PHERAstar v5.41.	BMG, UK	BMG Pherastar FSX platereader
Others
Shimadzu Prominence modular HPLC system	Shimadzu, Kyoto, Japan	Prominence modular HPLC system
Yarra 1.8μm SEC-x300 2.5mL column	Phenomenex, CA, US	00H-4743-E0-SS
TruPageTM Precast Gels 4-20%.	Sigma	PCG2008
PHERAstar ® FSX equipped with Time Resolved Fluorescence lasers and module, and TR337/665/620 and TR337/520/620modules	BMG, UK	PHERAstar ® FSX
ProxiPlate-384 Plus	PerkinElmer, MA, US	6008280
OptiPlate-384	PerkinElmer, MA, US	6007290
GE Amersham TyphoonTM	GE, US	GE Amersham TyphoonTM