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Example flow plots of in vitro cytotoxicity assay
Singlets are discriminated (FSC-H v. FSC-A) and CFSE-positive tumor cells are gated. Create a dead cell gate (APC-Cy7 positive) based on the samples that only contain tumor cells and no effector cells (basal). Apply dead cell gate to the non-transduced, control vector, and HER2 CAR-transduced expanded NK cell samples. Samples were acquired using the LSRFortessa Flow Cytometer and analyzed with FlowJo Software.
