Figure 2.

Reduced proliferation of spermatogonial stem cells (SSCs) caused by Rpl39l deletion

(A) SSCs from 2-week-old OG2 mice were sorted from intact cells (left panel, circled) using GFP expression and cKIT immunostaining (middle panel). Copy numbers of Rpl39l and Rpl39 transcripts were compared using quantitative RT-PCR (right panel). N = 3 experimental repeats, paired-sample Student’s t-test, ∗∗p < 0.01, ∗∗∗p < 0.001.

(B) GFRα1⁺ SSCs per unit length of seminiferous tubules in 2-week-old and adult mice. N ≥ 40 1-mm long seminiferous tubules.

(C) UCHL1⁺ spermatogonia per cross-section of 2-week-old mouse testes. N ≥ 183.

(D) Confocal images of testis sections of 2-week-old mice immunostained for UCHL1 (upper panels) or PLZF (lower panels). Scale bar: 50 μm.

(E) Percent of PLZF^High and PLZF^Low SSCs in total testicular cells from 2-week-old mice. N ≥ 7 testes.

(F) Ratio of PLZF^Low to PLZF^High SSCs in 2-week-old mice. N ≥ 7 testes.

(G) FACS for PLZF⁺ SSCs from 2-week-old mice.

(H) Percent of Ki67-labeled PLZF^Low SSCs from 2-week-old mice. N ≥ 3 testes.

(I) FACS for Ki67-labeled PLZF^Low SSCs from 2-week-old mice.

(J) TUNEL⁺ cells per cross-section containing TUNEL⁺ cells. N ≥ 203.

(K) Confocal images of TUNEL assays on mouse testis sections. Scale bar: 50 μm.

One-way ANOVA Tukey’s test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.