MaxCyte ATx mediated electroporation of RNP complex into iPSCs
(A) Prepare a mixture of MaxCyte HyClone buffer with cells and RNP complex, and ssODN if applicable. Transfer 50 μL of the mixture into the well of OC-100 Processing Assembly chamber, after opening the lid. Then close the lid after the sample has been added to the OC-100.
(B) Operation of the instrument. Select the desired electroporation protocol (Optimization Energy 8), and processing assembly (OC-100). Finally insert the OC-100 processing assembly and start electroporation.