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4D-Nucleofector mediated electroporation of RNP complex into iPSCs
(A) Prepare mixture of P4 primary buffer with cells and RNP complex, and ssODN if applicable. Transfer 20 μL of the mixture into one well of 16 well strips.
(B) Operation of the instrument. Select wells where your samples are, and then input pulse code (CA-137) and select solution (Primary Cell P4). Finally insert your 16 well strips and start electroporation.
