PCR reagents	Final concentration	Amount
Ultrapure H2O		Up to 50 μL
5 × PrimeSTAR GXL buffer	1 ×	10 μL
dNTPs Mix (2.5mM each)	0.2 mM	4 μL
Forward primer (10 μM)	0.3 μM	1.5 μL
Reverse primer (10 μM)	0.3 μM	1.5 μL
Genomic DNA	100–500 ng/50 μL	X μL
PrimeSTAR GXL DNA Polymerase	1.25 U/50 μL	1 μL
Total		50 μL