REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
iMatrix-511 silk (Laminin-511 E8)	Takara Bio	Cat#T311
Y-27632	Fujifilm Wako Pure Chemical Corporation	Cat#034-24024
StemFit® AK02N (or AK03N) media	Takara Bio	Cat#AK02N; AJ100
PBS (−)	Nacalai Tesque	Cat#14249-24
0.5 M EDTA	Nacalai Tesque	Cat#06894-14
Accutase	Sigma-Aldrich	Cat#A6964-500ML
TrypLE™ Select Enzyme (1×), no phenol red	Thermo Fisher Scientific	Cat#12563029
STEM-CELLBANKER® GMP grade	ZENOAQ	Cat#CB045
HyClone™ MaxCyte® Electroporation buffer	KIKO TECH	Cat#EPB-1
Recombinant SpCas9 protein	IDT, or Thermo Fisher Scientific	Cat#1081058, A36498
Lipofectamine Stem Transfection Reagent	Thermo Fisher Scientific	Cat#STEM00015
4-hydroxytamoxifen	Sigma-Aldrich	Cat#H7904
KOD-plus-Neo	Toyobo	Cat#KOD-401
PrimeSTAR® GXL DNA Polymerase	Takara bio	Cat#R050B
Critical commercial assays
MEGAshortscript™ T7 Transcription Kit	Thermo Fisher Scientific	Cat#AM1354
RNeasy MinElute Cleanup Kit	QIAGEN	Cat#74204
Wizard® SV Gel and PCR Clean-Up System	Promega	Cat#A9281
P4 Primary Cell 4D-Nucleofector™ X Kit S (32 RCT)	Lonza	Cat#V4XP-4032
Experimental models: Cell lines
1383D2 Human iPSCs, reprogrammed PBMC (LP_53, donor #40) isolated from a 36-years-old male donor by episomal vectors	Nakagawa et al. (2014)	From Dr. Nakagawa
DMD-iPSCs (CiRA00111 clone) from a male patient with Duchenne muscular dystrophy with exon 44 deletion (The sampling age is 1–9 years)	Li et al. (2015)	From Riken BRC https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=HPS0383
Ff-XT28s05 iPSCs were generated from a healthy male donor homozygous for the 3rd frequent HLA haplotypes in Japan (A∗24:02, B∗07:02, C∗07:02, DRB1∗01:01)	CiRA Foundation, Xu et al. (2019)	https://www.cira-foundation.or.jp/e/project/genome-edited.html
404C2 Human iPSCs, reprogrammed skin fibroblasts (HDF1388) isolated from a 36-years-old female donor by episomal vectors.	Okita et al. (2011)	From Dr. Okita, RRID:CVCL_DP92
Oligonucleotides
T7-DMDsgRNA1 forward primer: GAAA TTAATACGACTCACTATAgggtatcttacag gaactccGTTTTAGAGCTAGAAATA GCAAG	This Paper	N/A
T7-DMD-in55- g3-IVT forward primer: GAAA TTAATACGACTCACTATAggactttatagata tctcccaGTTTTAGAGCTAGAAATA GCAAG	This Paper	N/A
T7-DYSFgRNA2-IVT forward primer: GAAATT AATACGACTCACTATAggtactacacacact gacggGTTTTAGAGCTAGAAAT AGCAAG	This Paper	N/A
T7-sgRNA reverse primer: AAAGCACCGA CTCGGTGCCACTTTTTCAAGTTGATAAC GGACT AGCCTTATTTTAACTTGCTATTT CTAGCTCTAAAAC	This Paper	N/A
DMD1+loxPssODN (134-mer): AAGAC ATGGGGCTTCATTTTTGTTTTGCCTTT TTGGTATCTTACAGGAACATAACTTC GTATAGCATACATTATACGAAGTTAT TCCAGGATGGCATTGGGCAGCGGC AAACTGTTGTCAGAACATTGAATGCA	This Paper	N/A
DMD-in55- g3+loxP-ssODN (134-mer): TCA TTTGGAGGTAATTTGTTTGGAACAGTAT CAGACTTTATAGATATCTCATAACTTCGT ATAGCATACATTATACGAAGTTATCCATG GCTTGTGATAGAATATAAGGGCAATGC AAATGTAGAGTTTTTTGC	Kagita et al. (2021)	N/A
DMDexon55(45-55)check_dir5 primer: CCTCGGGTACACTGAAAGTTATGTG	This Paper	N/A
DMDexon45(45-55)check_rev5 primer: CACCACAGGCTTTAACTTCTGCCG	This Paper	N/A
Software and algorithms
CRISPRdirect	Naito et al. (2015)	https://crispr.dbcls.jp
CRISPOR	Concordet and Haeussler (2018)	http://crispor.tefor.net
ICE	SYNTHEGO	https://www.synthego.com/products/bioinformatics/crispr-analysis
TIDE	Brinkman et al. (2014)	http://shinyapps.datacurators.nl/tide/
Primer3	Untergasser et al. (2012)	https://bioinfo.ut.ee/primer3-0.4.0/
Primer-BLAST	Ye et al. (2012)	https://www.ncbi.nlm.nih.gov/tools/primer-blast/
Other
6-well plate	Greiner BIO-ONE	Cat#657160
12-well plate	Greiner BIO-ONE	Cat#665180
24-well plate	Greiner BIO-ONE	Cat#662160
96-well plate	Greiner BIO-ONE	Cat#655180
10 cm Cell Culture Dishes	Greiner BIO-ONE	Cat#664160-013
Falcon 5 mL Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap	Corning	Cat#352235
MaxCyte® OC-100 cuvette	Kiko tech	Cat#SOC-1
MaxCyte ATx	Kiko Tech	Cat#E-ATx
4D-Nucelofector™ Core Unit	Lonza	Cat#AAF-1002B
4D-Nucelofector™ X Unit	Lonza	Cat#AAF-1002X
Applied Biosystems™ Veriti™ 96-Well Thermal Cycler	Applied Biosystems	N/A
Genetic Analyzer Trade-in for 3500 Series System	Thermo Fisher Scientific	N/A
NanoDrop™ 2000c Spectrophotometers	Thermo Fisher Scientific	Cat#ND-2000C
Agilent 2200 TapeStation	Agilent Technologies	Cat#G2965A
BD FACS Aria™ II cell sorter	Becton, Dickinson and company	N/A