Table 2.
Primers used in real-time quantitative PCR.
| Gene | Gene bank ID | Primer sequence (5’-3′) |
|---|---|---|
| β-actin | NM_205518.1 | F:CCACCGCAAATGCTTCTAAAC R:AAGACTGCTGCTGACACCTTC |
| IL-1β | XM_015297469.1 | F:ACTGGGCATCAAGGGCTA R:GGTAGAAGATGAAGCGGGTC |
| IFN-γ | NM_205149.1 | F: CTTCCTGATGGCGTGAAGA R: GAGGATCCACCAGCTTCTGT |
| IL8 | NM_205018.1 | F:ATGAACGGCAAGCTTGGAGCTG R:TCCAAGCACACCTCTCTTCCATCC |
| TNF-α | NM_204267.1 | F: CCCCTACCCTGTCCCACAA R: TGAGTACTGCGGAGGGTTCAT |
| TLR-4 | NM_001030693.1 | F:GGATCTTTCAAGGTGCCACA R: CAAGTGTCCGATGGGTAGGT |
| NF-κB | NM_205129.1 | F:ACCCCTTCAATGTGCCAATG R:TCAGCCCAGAAACGAACCTC |
| MyD88 | NM_001030962.3 | F: TGCAAGACCATGAAGAACGA R: TCACGGCAGCAAGAGAGATT |
| Bcl-2 | NM_205339.2 | F:GAGTTCGGCGGCGTGATGTG R:CTCGGTCATCCAGGTGGCAATG |
| Caspase-3 | NM_204725.1 | F:TACCGGACTGTCATCTCGTTCAGG R:ACTGCTTCGCTTGCTGTGATCTTC |
| Bax | XM_204725 | F:TCCTCATCGCCATGCTCAT R:CCTTGGTCTGGAAGCAGAAGA |
| occludin | NM_205128.1 | F: ACGGCAGCACCTACCTCAA R: GGGCGAAGAAGCAGATGAG |
| claudin-1 | NM_001013611.2 | F: CATACTCCTGGGTCTGGTTGGT R: GACAGCCATCCGCATCTTCT |
| ZO-1 | NM_001301025.3 | F: CTTCAGGTGTTTCTCTTCCTCCTC R: CTGTGGTTTCATGGCTGGATC |
| mucin-2 | NM_001318434.1 | F: CCCTCACCCAGCCCGACTTC R: GCCGTTGGTGGAGGTGTTACAG |
Abbreviations: F, forward; R, reverse.