. 2021 Oct 13;101(1):101541. doi: 10.1016/j.psj.2021.101541

Table 6.

Effects of different proportions of naturally oxidized oil on relative mRNA expression of jejunum inflammation and apoptosis cytokines genes of broilers.

Parameter¹	OXA	OXB	OXC	OXD	OXE	SEM	P-value	P-linear	P-quadratic
IL-1β	1.00^b	1.45^ab	1.84^ab	2.22^a	2.17^a	0.09	0.047	0.568	0.109
IFN-γ	1.00^b	0.99^b	1.14 ^b	1.89^a	1.12^b	0.11	0.043	0.057	0.499
IL8	1.00	1.05	0.90	1.03	0.59	0.04	0.267	0.114	0.262
TNF-α	1.00^b	1.23^ab	1.21 ^ab	1.80^a	1.30^ab	0.04	0.045	0.39	0.073
TLR4	1.00	0.54	0.64	0.55	0.78	0.02	0.166	0.35	0.038
NF-κB	1.00^b	1.28^ab	1.03^b	1.74^a	0.97^b	0.10	0.042	0.064	0.552
MyD88	1.00^b	1.22^b	1.45^ab	1.94^a	1.47^ab	0.11	0.041	0.271	0.386
Bcl-2	1.00	1.11	0.66	0.73	0.74	0.13	0.397	0.15	0.637
Caspase-3	1.00	0.95	0.77	0.98	0.93	0.08	0.507	0.751	0.289
Bax	1.00^b	0.91^b	1.26^a	1.17^ab	0.94^b	0.04	0.030	0.189	0.031

Abbreviations: Bax, Bcl2-associated X; Bcl-2, B-cell lymphoma-2; Caspase-3, cysteinyl aspartate specific proteinase-3; IL-1β, interleukin-1β; IFN-γ, interferon-γ; IL-8, interleukin-8; MyD88, myeloid differentiation factor88; NF-κB, nuclear factor kappa B; OXA, basal diet with 0% naturally oxidized oil; OXB, basal diet with 25% naturally oxidized oil; OXC, basal diet with 50% naturally oxidized oil; OXD, basal diet with 75% naturally oxidized oil; OXE, basal diet with 100% naturally oxidized oil; TNF-α, tumor necrosis factor α; TLR-4, toll-like receptor-4.

^ab

Different letters in the same row indicate significant differences (P < 0.05), and the same letter means no significant difference (P > 0.05).

Gene relative expression in different treatments of broilers (N = 6).