Antibodies |
|
SMN clone 8 |
BD Transd Lab |
610646, RRID:AB_397973
|
Tubulin DM1A |
Sigma |
T9026, RRID:AB_1965960
|
p53 |
Leica Novocastra |
NCL-p53-CM5p, RRID:AB_563933
|
p-p53S15
|
Cell Signaling |
9284 (Lots: 12,15), RRID:AB_331464
|
VGluT1 |
Synaptic Systems |
135 304, RRID:AB_887878
|
Synaptophysin |
Synaptic Systems |
101-004, RRID:AB_1210382
|
Neurofilament |
Millipore |
AB1987, RRID:AB_91201
|
ChAT |
Millipore |
AB144P, RRID:AB_2079751
|
Bungarotoxin |
Invitrogen |
B35451, RRID:AB_2617152
|
Kv2.1 |
Neuromab |
K89/34 concent., RRID:AB_2877280
|
Parvalbumin |
Synaptic Systems |
195 004, RRID:AB_2156476
|
SmB |
Santa Cruz |
Sc-130670, RRID:AB_2193856
|
VGAT |
Synaptic Systems |
131004, RRID:AB_887873
|
488 Anti-rabbit |
Jackson |
711-545-152, RRID:AB_2313584
|
488 Anti-goat |
Jackson |
705-545-147, RRID:AB_2336933
|
488 Anti-chicken |
Jackson |
703-545-155, RRID:AB_2340375
|
Cy3 Anti-rabbit |
Jackson |
711-165-152, RRID:AB_2307443
|
Cy3 Anti-mouse |
Jackson |
715-165-150, RRID:AB_2340813
|
Cy3 Anti-goat |
Jackson |
705-165-147, RRID:AB_2307351
|
Cy5 Anti-goat |
Jackson |
705-175-147, RRID:AB_2340415
|
Cy5 Anti-guinea pig |
Jackson |
706-175-148, RRID:AB_2340462
|
HRP Anti-mouse |
Jackson |
115-035-044, RRID:AB_2338503
|
HRP Anti-rabbit |
Jackson |
111-035-003, RRID:AB_2313567
|
|
Chemicals, peptides, and recombinant proteins |
|
Pifithrin-α |
Merck |
P4359 |
|
Experimental models: Organisms/strains |
|
SMNΔ7 |
Jackson |
#005025 |
Taiwanese |
Jackson |
#005058 |
Smn2B/-
|
Bowerman et al. (2012) |
Provided by Dr. Kothari |
|
Oligonucleotides |
|
RT-qPCR p53
|
Simon et al. (2017) |
N/A |
RT-qPCR Cdkn1a
|
Ruggiu et al. (2012) |
N/A |
RT-qPCR Perp1
|
Van Alstyne et al. (2018) |
N/A |
RT-qPCR Gtse1
|
Van Alstyne et al. (2018) |
N/A |
RT-qPCR Stas Aber |
Lotti et al. (2012) |
N/A |
RT-qPCR H1c pre |
Van Alstyne et al., 2018 |
N/A |
RT-qPCR Chodl
|
Van Alstyne et al. (2018) |
N/A |
RT-qPCR Gapdh
|
Ruggiu et al. (2012) |
N/A |
RT-PCR Mdm2 E2-5 |
Van Alstyne et al. (2018) |
N/A |
RT-PCR Mdm4 E5-8 |
Van Alstyne et al. (2018) |
N/A |
PCR primers for genotyping SMNΔ7
|
Simon et al. (2017) |
N/A |
PCR primers for genotyping Taiwanese
|
Riessland et al. (2010) |
N/A |
PCR primers for genotyping Smn2B/-
|
Bowerman et al. (2012) |
N/A |
|
Software and algorithms |
|
LAS X |
Leica |
N/A |
Prism 9 |
GraphPad |
N/A |