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Ligament type I collagen has elevated N-telopeptide cross-linking lysine hydroxylation compared to tendon. LC-MS profiles of collagenase-digested adult ACL (A), Fetal ACL (B), Adult QT (C) and Fetal QT (D). (A) Adult ligaments had the highest level of hydroxylysine (~10152+) at the N-telopeptide cross-linking site of all tissues tested. A small population of peptide containing hydroxylysine + oxidized methionine (~10232+) was observed in adult tissues.
