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. 2021 Oct 27;297(6):101346. doi: 10.1016/j.jbc.2021.101346

Figure 5.

Figure 5

ComR-F171 and Y174 mutants.A, maximum specific luciferase activity (% RLU × OD600−1) of S. thermophilus ΔcomS reporter strains producing ComRSth WT (blue), the double mutant ComR-F171A-Y174A (red) or the single mutants ComR-F171A (green), ComR-F171L (gray), ComR-Y174A (orange), or ComR-Y174L (dark gray) in the absence (−XIP) or presence (+XIP) of XIPSth WT (100 nM). B, dose response of PcomS activity upon XIPSth WT (solid lines) or XIP-A5M (dotted lines) addition to reporter strains carrying ComRSth WT (blue), ComR-F171A-Y174A (red), ComR-F171A (green), or ComR-Y174A (orange). The monitoring and normalization of luciferase activity were performed as in Figure 2. C, fold changes in EC50 calculated from the dose response curves shown in panel B. The EC50 of the double mutant ComR-F171A-Y174A for XIPSth is used as reference (R) and normalized to 1. Decrease in fold change is representative of an increase in EC50. In A, B, and C, experimental values represent the mean ± SD of at least three independent replicates. In A, significant differences (ComRSth WT as reference in the presence or absence of XIP) calculated by Student unpaired t test are indicated (∗∗∗∗p-value < 0.0001, ∗∗∗p-value < 0.001; ∗p-value < 0.05).