Fig. 1.

Characterization of StemCell Keep (SCK)-cryopreserved human induced pluripotent stem (hiPS) cells.

(a) Representative images of alkaline phosphatase (AP) + staining of SCK- or DAP213 (DAP)-cryopreserved hiPS cells. (b) Number of AP + colonies that generated from SCK- or DAP-cryopreserved hiPS cells. *p = 0.002 (c) Representative images of immunofluorescence staining of SCK- or DAP-cryopreserved hiPS cells. Oct 3/4-FITC staining (green), i and vii; Nanog-FITC staining (green), iv and x; DAPI, 4,6- diamidino-2-phenylindole, staining (blue), ii, v, viii and xi; bright-field images, iii, vi, ix and xii. Scale bar: 100 μm. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)