Table 3b.

DAVID analysis for genes of DAP-cryopreserved hiPS cells classified under ‘apoptosis’ with a difference in expression of more than 1.5-fold compared with the non-frozen hiPS cells (p < 0.05).

No.	Category	Term	Count	%	PValue	Genes	List Total	Pop Hits	Pop Total	Fold Enrichment	Bonferroni	Benjamini	FDR
1	GOTERM_BP_DIRECT	GO:0032464∼positive regulation of protein homooligomerization	2	15.38	0.005	BID BMF	11	9	16792	339.23	0.722	0.665	0.665
2	GOTERM_BP_DIRECT	GO:0043065∼positive regulation of apoptotic process	3	23.08	0.013	BCL2A1 BID BMF	11	300	16792	15.27	0.956	0.665	0.665
3	GOTERM_BP_DIRECT	GO:0001836∼release of cytochrome c from mitochondria	2	15.38	0.014	BCL2A1 BID	11	23	16792	132.74	0.962	0.665	0.665
4	GOTERM_BP_DIRECT	GO:0090200∼positive regulation of release of cytochrome c from mitochondria	2	15.38	0.017	BID BMF	11	28	16792	109.04	0.981	0.665	0.665
5	GOTERM_BP_DIRECT	GO:0045944∼positive regulation of transcription from RNA polymerase II promoter	4	30.77	0.018	CD28 IL10 PTH HIPK2	11	981	16792	6.22	0.985	0.665	0.665
6	GOTERM_BP_DIRECT	GO:1900740∼positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway	2	15.38	0.018	BID BMF	11	30	16792	101.77	0.986	0.665	0.665
7	GOTERM_BP_DIRECT	GO:2001244∼positive regulation of intrinsic apoptotic signaling pathway	2	15.38	0.019	BID BMF	11	33	16792	92.52	0.991	0.665	0.665
8	GOTERM_BP_DIRECT	GO:0008630∼intrinsic apoptotic signaling pathway in response to DNA damage	2	15.38	0.028	BCL2A1 MLH1	11	47	16792	64.96	0.999	0.761	0.761
9	GOTERM_BP_DIRECT	GO:0006468∼protein phosphorylation	3	23.08	0.029	PRKCA HIPK2 STK17B	11	456	16792	10.04	0.999	0.761	0.761
10	GOTERM_BP_DIRECT	GO:0097190∼apoptotic signaling pathway	2	15.38	0.041	CD28 PRKCA	11	71	16792	43.00	1.000	0.992	0.992
11	KEGG_PATHWAY	hsa05143:African trypanosomiasis	2	15.38	0.033	IL10 PRKCA	8	33	6879	52.11	0.958	0.887	0.887
12	KEGG_PATHWAY	hsa05330:Allograft rejection	2	15.38	0.037	CD28 IL10	8	37	6879	46.48	0.971	0.887	0.887
13	KEGG_PATHWAY	hsa04672:Intestinal immune network for IgA production	2	15.38	0.047	CD28 IL10	8	47	6879	36.59	0.989	0.887	0.887
14	KEGG_PATHWAY	hsa05320:Autoimmune thyroid disease	2	15.38	0.052	CD28 IL10	8	52	6879	33.07	0.993	0.887	0.887