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FACS-based enrichment of V. natriegens L-lysine producers harboring a LysG-based biosensor. A) Fluorescence per cell distribution per enrichment step (n = 10,000). Boxplots show median and first and third quartiles (lower and upper hinges); 300,000 cells of the top 5% fluorescent cells were sorted after each cultivation. B) Extracellular L-lysine concentration of 96 cultures from sorted single isolates.
