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. 2021 Nov 11;13:e00187. doi: 10.1016/j.mec.2021.e00187

Table 1.

Bacterial strains used in this study.

Strain/plasmid Genotype and relevant characteristic Reference
V. natriegens ATCC 14048; DSM 759 Wild type German Collection of Microorganisms and Cell Cultures, (Payne, 1958)
E. coli DH5a supE 44 ΔlacU169 (φ80lacZDM15) hsdR17 recA1 endA1 gyrA 96 thi-1 relA1 Invitrogen (Karlsruhe, Germany)
pBR322 AmpR, TetR, Bolivar et al. (1977)
pBR322-eYFP AmpR, pBR322 derived backbone used for LysG sensor construction This study
pBR322-J23100-Lrp-PbrnFE-eYFP AmpR, pBR322 derived Lrp biosensor, Lrp expressed under the J23100 promoter This study
pBR322-J23101-Lrp-PbrnFE-eYFP AmpR, pBR322 derived Lrp biosensor, Lrp expressed under the J23101 promoter This study
pBR322-J23106-Lrp-PbrnFE-eYFP AmpR, pBR322 derived Lrp biosensor, Lrp expressed under the J23106 promoter This study
pBR322-J23108-Lrp-PbrnFE-eYFP AmpR, pBR322 derived Lrp biosensor, Lrp expressed under the J23108 promoter This study
pBR322-J23110-Lrp-PbrnFE-eYFP AmpR, pBR322 derived Lrp biosensor, Lrp expressed under the J23110 promoter This study
pBR322-J23100-LysG-PlysE-eYFP AmpR, pBR322 derived LysG biosensor, LysG expressed under the J23100 promoter This study
pBR322-J23101-LysG-PlysE-eYFP AmpR, pBR322 derived LysG biosensor, LysG expressed under the J23101 promoter This study
pBR322-J23104-LysG-PlysE-eYFP AmpR, pBR322 derived LysG biosensor, LysG expressed under the J23104 promoter This study
pBR322-J23106-LysG-PlysE-eYFP AmpR, pBR322 derived LysG biosensor, LysG expressed under the J23106 promoter This study
pBR322-J23108-LysG-PlysE-eYFP AmpR, pBR322 derived LysG biosensor, LysG expressed under the J23108 promoter This study
pBR322-J23110-LysG-PlysE-eYFP AmpR, pBR322 derived LysG biosensor, LysG expressed under the J23110 promoter This study