Table 2.
Relevant mutations of isolated V. natriegens amino acid producer strains. Total SNPs for each strain are indicated in brackets.
| # | Phenotype | Relevant mutations |
|---|---|---|
| H1 | L-histidine producer (23 SNPs) | serine ammonia-lyase (V395I) |
| IMP dehydrogenase (L433F) | ||
| carA, carbamoyl phosphate synthetase (P358L) | ||
| A1 | L-arginine producer (35 SNPs) | agmatine deiminase (P77L) |
| potD, ABC transporter substrate-binding protein (L15F) | ||
| acetolactate synthase large subunit (Y296C and W486*) | ||
| threonine synthase (A186V) | ||
| upstream of glutamate synthase (G to A and G to A) | ||
| L1 | L-lysine producer (23 SNPs) | dapA, 4-hydroxy-tetrahydrodipicolinate synthase (H56Y) |
| DUF1338 domain-containing protein (V92I) | ||
| L2 | L-lysine improved producer (2 SNPs) | All mutations of L1 |
| DNA topoisomerase I (S500P) |