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. 2021 Oct 20;20:100167. doi: 10.1016/j.mcpro.2021.100167

Fig. 3.

Fig. 3

Discrimination between GlcNAc and GalNAc through HexNAc fragment ion ratio distributions.A, kernel density plots for the distribution of GlcNAc/GalNAc ratios [(138 + 168)/(126 + 144)], as determined by Halim et al. (46) for control proteins and conditions. Inset is a zoom of the higher ratios for all values highlighted. Comp, computational removal of MS2 spectra containing diagnostic ions for 366 and 274; N, the number of MS2 spectra used to derive ratios; PGF, PNGase F treatment; Sia, sialidase treatment. B, kernel density plots for the distribution of 138/144 peaks across the same samples in Figure 3A. Inset is a zoom of the higher ratios. Abbreviations are the same from Figure 3A.