Figure 3.

Knockdown of PHF5A induces genome-wide alternative splicing events

(A) Heatmap displaying the deregulated genes by RNA-seq of CRC cells upon PHF5A knockdown. (B) Gene ontology of PHF5A-regulated gene expression events. Fisher’s exact p values were plotted for each category. (C) GSEA of gene signature reported to be associated with poor survival in PHF5A knockdown cells versus control cells. NES, normalized enrichment score. (D) Quantification of the different AS events affected by PHF5A. (E) The relative fraction of each AS event affected positively or negatively by PHF5A. (F) Skipped (green) and included (red) PHF5A-regulated cassette exons plotted by PSI. (G) Gene ontology of PHF5A-regulated AS targets. Fisher’s exact p values were plotted for each enriched functional category.