Figure 1.

MDSCs accumulate in B16-F10 tumor-bearing mice

(A) Representative dot plots of CD11b⁺Gr-1⁺ MDSCs in the bone marrows (left two panels) and spleen (right two panels) of naive mice or B16-F10 tumor-bearing mice.

(B) Unpaired Student’s t test was done for statistical analyses of MDSC ratio.

(C) MDSCs of bone marrow from naive and B16-F10 tumor-bearing mice labeled by CD11b (red), Gr1 (green), and nucleus (blue) to detect the expression of MDSC makers.

(D) Volcano plot of the differentially expressed proteins of MDSCs between native mice and B16-F10 tumor-bearing mice (n = 3).

(E) Enrichment analysis of differentially expressed proteins by Metascape (https://metascape.org).

(F–H) Immunosuppressive function analysis of MDSCs including ROS level, NO production, and Arg1 activity. All statistical data are represented as mean ± SEM and Unpaired Student’s t test was done for statistical analyses. ∗p < 0.05, ∗∗p < 0.01.