Figure 3.

Doxycycline inhibits the promoting capability of MDSCs for VM formation in vitro

(A) Flow cytometric analysis of the proportion of MDSCs in bone marrow from C57BL/6 tumor-bearing mice treated by different doses of doxycycline and 5-FU.

(B) Statistical analysis of the ratio of MDSCs.

(C) Flow cytometric analysis of the proportion of PMN-MDSCs (CD11b⁺Ly6G⁺Ly6C^low) and M-MDSCs (CD11b⁺Ly6G⁻Ly6C^high) in bone marrow from C57BL/6 tumor-bearing mice treated by different doses of doxycycline and 5-FU.

(D) Statistical analysis of the ratio of MDSCs subsets.

(E) Representative CFSE histograms of PMN-MDSCs.

(F) Statistical analysis of the ratio of proliferating cells.

(G) Representative dot plots for apoptosis of T cells after DOX treatment. (H) Statistical analysis of the apoptosis ratio of T cells.

(I) Western blot to detect the levels of Arg-1 and iNOS in MDSCs after different doses of DOX treatment.

(J) Schematic showing the co-culture of B16 and MDSCs with or without DOX treatment.

(K–L) Representative images and statistical analysis of tube formation to evaluate the ability of co-cultured cells.

(M) Immunofluorescence image to detect the expression of VE-cadherin of B16 cells. All statistical data are represented as mean ± SEM. Unpaired Student’s t test was done for statistical analyses. ∗∗p < 0.01, ∗∗∗p < 0.001.