Quality control and output
(A) Overlay of automatically detected cells and corresponding image channel for visual validation of object detection. Shown is data of the example dataset (ChatCre;Ai14tdTomato); green: channel 1 (555nm), red: objects detected using Ilastik.
(B) Overlay of the atlas template (green) and the experimental example dataset (red, Neurotrace channel) after atlas alignment.
(C) SpinalJ output graphs like this heatmap montage of cell densities within channel 1 of the example dataset provide a quick visual summary of the data. Scale: 500μm.