Figure 4.

The homemade CS-MAP setup

(A) Photograph of the assembled device. The magnet and the PCR rack are taped to the top of the container. A 1 mL tip is placed next to the flat surface of the magnet.

(B) Photograph of the device during immunomagnetic immobilization of mitochondria to the tip wall closest to the magnet. Note that the micropipette must remain attached to the tip to prevent the loss of the sample prior to forming a magnetically trapped pellet.

(C) Photograph of the device during the washing step. The 25 mL pipette is placed into the opening of the 1 mL tip and PEB is released by gravity flow by carefully unplugging the pipettor.