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. 2021 Nov 16;2(4):100952. doi: 10.1016/j.xpro.2021.100952

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Quantification of mKate2-labelled IP^ed mitochondria obtained from SJZ47 myo-3p::TOMM-20::mKate2::HA

IP^ed mitochondria were attracted to the edge of the sample using a magnet.

(A and B) An approximative and manual assembling of a crescent shape formed by the beads and mKate2-labelled ^HA-taggedmitochondria is shown. The white dotted line indicates the front of the crescent.

(C–F) A portion of the crescent shape structure formed by the beads and mitochondria is shown. (C and D) mKate2-labelled ^HA-taggedmitochondria bound to anti-HA magnetic beads. (E and F) Anti-HA magnetic beads saturated with HA-peptide block the binding of the ^HA-taggedmitochondria (negative control). Mitochondria were quantified using FIJI and the Grid plugin (shown as a yellow grid). Observations were performed with a fluorescence compound microscope using DIC and red channel (excitation wavelength 587 nm, emission wavelength 610 nm), at 20× magnification, non-immersed. Scale bar: 100 μm.