Table 1.

Size of starting population based on desired application

Application	Starting population	Quantity of 100 mm NGM plate
Nucleic acid analyses (Quantitative PCR [qPCR], PCR) as shown previously in Ahier et al. (2018) and (2021).	10,000 to 20,000 animals	1 to 4 (according to Escherichia coli OP50 quantity on dishes).
Protein analyses (e.g., Western Blot) as shown previously in Ahier et al. (2018).	50,000 to 100,000 animals	5 to 20 (according to E. coli OP50 quantity on dishes)
Functional analyses (e.g., Seahorse metabolic assay) as shown previously in Ahier et al. (2018).	50,000 to 100,000 animals	5 to 20 (according to E. coli OP50 quantity on dishes)