PEB: Phosphate EDTA BSA Buffer

Reagent	Final concentration	Amount
10× PBS	1×	20 mL
BSA	1%	2 g
0.5 M EDTA	2 mM	0.8 mL
ddH2O	n/a	Up to 200 mL
Total	n/a	200 mL

Add 1 tablet of protease inhibitor cocktail per 100 mL of buffer prior to use. PEB with protease inhibitor cocktail can be stored at −20°C for a maximum of 6 months. PEB used for washing in the immunomagnetic purification step should be prepared without protease inhibitor cocktail, kept on ice for 1 h, and used within the same day.