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MIB: Mitochondrial Isolation Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| KCl | 50 mM | 0.744 g |
| Mannitol | 110 mM | 4 g |
| Sucrose | 70 mM | 4.792 g |
| 0.5 M EDTA | 0.1 mM | 0.04 mL |
| 1 M Tris HCl pH 7.4 | 5 mM | 1 mL |
| ddH2O | n/a | Up to 200 mL |
| Total | n/a | 200 mL |
Add 1 tablet of protease inhibitor cocktail per 100 mL of solution prior to use. MIB with protease inhibitor cocktail can be stored at −20°C for a maximum of 6 months.
