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. 2021 Nov 16;2(4):100952. doi: 10.1016/j.xpro.2021.100952

M9 buffer

Reagent Final concentration Amount
KH2PO4 3 g 22 mM
Na2HPO4 6 g 42 mM
NaCl 5 g 86 mM
MgSO4 0.25 g 1 mM
ddH2O n/a Up to 50 mL
Total n/a 50 mL

Store at 22°C–25°C for a maximum of 6 months.