RIPA Buffer: Radioimmunoprecipitation Assay Buffer

Reagent	Final concentration	Amount
1 M Tris HCl pH 8	10 mM	1 mL
0.5 M EDTA	1 mM	0.2 mL
Triton X-100	1%	1 mL
Sodium deoxycholate	0.1%	0.1 g
SDS	0.1%	0.1 g
NaCl	140 mM	0.8 g
ddH2O	n/a	Up to 100 mL
Total	n/a	100 mL

Store at 4°C for a maximum of 6 months. Add 1 tablet of protease inhibitor cocktail per 100 mL of buffer immediately before use.