TBST: Tris-buffered saline with 0.1% Tween-20 detergent
| Reagent | Final concentration | Amount |
|---|---|---|
| TBS 10× pH 7.5 | 1× | 100 mL |
| Tween-20 | 0.1% | 1 mL |
| ddH2O | n/a | Up to 1 L |
| Total | n/a | 1 L |
Store at 22°C–25°C for a maximum of 6 months.
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