REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal Anti-HA magnetic beads (100 μg)	Pierce	Cat# 88837; RRID:AB_2861399
Rabbit Anti-HA-Tag Monoclonal Antibody (C29F4; 1:5000)	Cell Signaling Technology	Cat# 3724, RRID:AB_1549585
Mouse monoclonal anti-MTCO1 [1D6E1A8] (1:1000)	Abcam	Cat# ab14705; RRID:AB_2084810
Mouse monoclonal anti-ATPB [3D5] (1:1000)	Abcam	Cat# ab14730; RRID:AB_301438
Mouse monoclonal anti-HSP-60 [HSP60] (1:250)	DSHB (Hadwiger et al., 2010)	Cat# HSP60; RRID:AB_10572252
Mouse monoclonal anti-ELT-2 [455-2A4] (1:200)	DSHB (Hadwiger et al., 2010)	DSHB Cat# 455-2A4, RRID:AB_2618114
Mouse monoclonal anti-DLG-1 [DLG1] (1:100)	DSHB (Hadwiger et al., 2010)	DSHB Cat# DLG1, RRID:AB_2314321
IRDye 800CW goat anti-rabbit (1:20000)	LI-COR Biosciences	Cat# 925-32211; RRID:AB_2651127
IRDye 680RD goat anti-mouse (1:20000)	LI-COR Biosciences	Cat# 925-68070; RRID:AB_2651128
Bacterial and virus strains
Escherichia coli OP50	Caenorhabditis Genetics Center (CGC)	OP50
Chemicals, peptides, and recombinant proteins
Proteinase K	Sigma-Aldrich	V3021
SIGMAFAST Protease Inhibitor Cocktail Tablets	Sigma-Aldrich	S8830
Mannitol	Sigma-Aldrich	M4125
Sucrose	Sigma-Aldrich	S7903
BSA	Bovogen Biologicals	BSAS 0.1
PBS 10×	Lonza	17-517Q
KCl	Sigma-Aldrich	P9541
NP40	Sigma-Aldrich	I8896
Tween 20	Sigma-Aldrich	P9416
Gelatin from bovine skin	Sigma-Aldrich	G9391
HA peptide	Sigma-Aldrich	I2149
Glycogen	Thermo Fisher Scientific	AM9510
Phenol-chloroform-isoamyl alcohol	Sigma-Aldrich	77617
4× Laemmli Sample Buffer	Bio-Rad	1610747
GeneRuler DNA Ladder Mix	Thermo Fisher Scientific	SM0331
Experimental models: Organisms/strains
C. elegans: strain N2 (wild-type) L4 stage, hermaphrodite	Caenorhabditis Genetics Center (CGC)	N2 (strain) WormBase: WBStrain00000001
C. elegans: strain SJZ47 (foxSi16 [myo-3p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I) L4 stage, hermaphrodites	Caenorhabditis Genetics Center (CGC)	SJZ47 (Ahier et al., 2018)
C. elegans: strain SJZ216 (foxSi44 [rgef-1p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I) L4 stage, hermaphrodites	Caenorhabditis Genetics Center (CGC)	SJZ216 (Ahier et al., 2018)
C. elegans: strain SJZ213 (foxSi41 [dpy-7p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I) L4 stage, hermaphrodites	Caenorhabditis Genetics Center (CGC)	SJZ213 (Ahier et al., 2018)
C. elegans: strain SJZ204 (foxSi37 [ges-1p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I) L4 stage, hermaphrodites	Caenorhabditis Genetics Center (CGC)	SJZ204 (Ahier et al., 2018)
C. elegans: strain SJZ328 (foxSi75 [eft-3p::tomm-20::mKate2::HA::tbb-2 3′ UTR] I) L4 stage, hermaphrodites	Caenorhabditis Genetics Center (CGC)	SJZ328 (Ahier et al., 2018)
Oligonucleotides
CD15 5′-agcgtcatttattgggaagaagac-3′	(Ahier et al., 2018)	N/A
AA113 5′-ccttccaaatactccgtctgc-3′	(Ahier et al., 2018)	N/A
ges-1F1 5′-gctctccgcatgctaatgaatacc-3′	(Ahier et al., 2018)	N/A
ges-1R1 5′-ctaactggtctccattctacatcttc-3′	(Ahier et al., 2018)	N/A
AA178 5′-cataccgaataaacatcagggtaatct-3′	(Ahier et al., 2021)	N/A
AA179 5′-acgggtgttacactatgatgaaga-3′	(Ahier et al., 2021)	N/A
SZ35 5′-aggctaagccggggtaagtt-3′	(Ahier et al., 2021)	N/A
SZ36 5′-gccaaaagcttaaactgcgg-3′	(Ahier et al., 2021)	N/A
Software and algorithms
Zen2	Zeiss	version 2.0.0.0
FIJI	(Schindelin et al., 2012)	1.52p
GraphPad Prism v9.1.0	GraphPad Software	https://www.graphpad.com/
Rotor-Gene Q Pure Detection software v2.3.1	QIAGEN	https://www.qiagen.com/
Microsoft Office 365	Microsoft Corporation	https://www.microsoft.com/en-au/microsoft-365
Adobe Illustrator CC 2021	Adobe	https://www.adobe.com/
Other
Z2 imager microscope with an Axiocam 506 mono camera	Zeiss	Zeiss
Thermo Scientific S1 Pipet Filler	Thermo Fisher Scientific	S1
Serological pipet 25 mL	Falcon	357525
Sigma Dounce tissue grinder pestle - small clearance (B), working volume 7 mL	Sigma-Aldrich	P1235
Dounce tissue grinder tube working volume 7 mL	Sigma-Aldrich	T0566
NanoPhotometer	Implen	N60
16-Tube SureBeads™ Magnetic Rack	Bio-Rad	1614916
Rotor-Gene machine	QIAGEN	NA
Odyssey Fc Imager	LI-COR	N/A