TABLE 1.
Strains used in this studya
| Strain | Genotype | Background | Source or reference |
|---|---|---|---|
| FYBL1-23D | MATα flo8-1 KSS1 ura3-Δ851 trp1Δ63 his3Δ200 | S288C | B. Dujon |
| FY839 | MATa flo8-1 KSS1 ura3-52 his3Δ200 leu2Δ1 | S288C | F. Winston |
| FY1679-28C | MATa flo8-1 KSS1 ura3-52 trp1Δ63 leu2Δ1 his3Δ200 | S288C | B. Dujon |
| JC297 | MATα FLO8 (?) KSS1 ura3-167 trp1::hisG his3Δ200 Ty1his3AI-270 | J. Curcio (13) | |
| JC242 | MATα FLO8 (?) KSS1 ura3-167 his3Δ200 Ty1his3AI-242 | J. Curcio (16) | |
| L5684 | MATa FLO8 KSS1 ura3-52 leu2::hisG | Σ1278b | G. Fink |
| L5685 | MATa FLO8 KSS1 ura3-52 trp1::hisG | Σ1278b | G. Fink |
| LV50 | MATa flo8-1 KSS1 ura3-Δ851 his3Δ200 trp1Δ63 leu2Δ1 lys2Δ202 | S288C | This study |
| LV69 | MATα flo8-1 KSS1 lys2::his3Δ4 ura3-Δ851 trp1Δ63 his3Δ200 | S288C | This study |
| LV101 | MATα flo8-1 KSS1 ste12Δ1::TRP1 ura3-Δ851 trp1Δ63 his3Δ200 | S288C | This study |
| LV105 | MATa flo8-1 KSS1 ste12Δ1::TRP1 ura3-52 trp1Δ63 leu2Δ1 his3Δ200 | S288C | This study |
| LV126 | MATα flo8-1 KSS1 tec1Δ1::TRP1 ura3-Δ851 trp1Δ63 his3Δ200 | S288C | This study |
| LV128 | MATa flo8-1 KSS1 tec1Δ1::TRP1 ura3-Δ851 his3Δ200 trp1Δ63 leu2Δ1 lys2Δ202 | S288C | This study |
| LV148 | MATa FLO8 KSS1 ura3-52 trp1::hisG his3Δ::TRP1 | Σ1278b | This study |
| LV150 | MATα flo8-1 KSS1 lys2::ROAM-his3Δ4-1 ura3-Δ851 trp1Δ63 his3Δ200 | S288C | This study |
| LV237 | MATα flo8-1 kss1::KanMX ura3-Δ851 trp1Δ63 his3Δ200 | S288C | This study |
| LV247 | MATa flo8-1 kss1::KanMX ura3-52 his3Δ200 leu2Δ1 | S288C | This study |
a
The S288C strains are isogenic to the S288C strain used as a source of DNA for the European Union Yeast Genome Sequencing program. The TY1A-lacZ fusions have been introduced in FYBL1-23D. S288C diploid strains are unable to form filaments due to the flo8-1 mutation. Diploid strains obtained by mating Σ1278b haploid strains with S288C haploid strains or with JC242 or JC297 haploid strains form filaments well.