TABLE 2.
Expression of native Ty1 elements in haploid and diploid cellsa
| TY1A-lacZ fusion(s) | Color in the presence of X-Gal
|
|
|---|---|---|
| Haploid | Diploid | |
| DR3, DR5, ER2, LR2, and ML1 | − | − |
| DR1, GR2, GR3, JR1, LR1, MR1, MR2, NL1, OL, and PR2 | + | − |
| A, NL2, and OR | + | + |
| DR4, DR6, LR3, ML2, PL, and PR3 | ++ | − |
| BL, BR, ER1, GR1, JR2, and PR1 | ++ | + |
| LR4 | ++ | ++ |
a
TY1A-lacZ expression was determined on patches of cells by filter assays. −, white; +, light blue; ++, blue. The nomenclature of the Ty1 elements follows the sequence annotation established by MIPS (http://www.mips.biochem.mpg.de/), e.g., ER2 is the second element on the right arm of chromosome V). On chromosome IV, the Ty1 element previously identified as DR2 by MIPS is a Ty2 element (http://www.public.iastate.edu/∼voytas/). Haploid, TY1A-lacZ fusions introduced into FYBL1-23D by recombination (see Materials and Methods); diploid, FYBL1-23D derivatives carrying the TY1A-lacZ fusions mated with FY839.