Reagent | Final concentration | Amount |
---|---|---|
Advanced DMEM/F-12 | n/a | 485 mL |
Glutamax (100×) | 1× | 5 mL |
Penicillin-Streptomycin (10,000 U/mL) | 1000 U/mL | 5 mL |
HEPES (1 M) | 10 mM | 5 mL |
Total | n/a | 500 mL |
It can be stored at 4°C up to 1 month.
Reagent | Final concentration | Amount |
---|---|---|
Advanced DMEM/F-12 | n/a | 485 mL |
Glutamax (100×) | 1× | 5 mL |
Penicillin-Streptomycin (10,000 U/mL) | 1000 U/mL | 5 mL |
HEPES (1 M) | 10 mM | 5 mL |
Total | n/a | 500 mL |
It can be stored at 4°C up to 1 month.