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. 2021 Nov 17;2(4):100935. doi: 10.1016/j.xpro.2021.100935

Mouse organoid basic medium

Reagent Final concentration Amount
Advanced DMEM/F-12 n/a 485 mL
Glutamax (100×) 1× 5 mL
Penicillin-Streptomycin (10,000 U/mL) 1000 U/mL 5 mL
HEPES (1 M) 10 mM 5 mL
Total n/a 500 mL

It can be stored at 4°C up to 1 month.