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Co-culture medium
| Reagent | Final concentration | Amount |
|---|---|---|
| Mouse organoid basic medium | n/a | 19.53 mL |
| 50× B27 Supplement | 1× | 400 μL |
| 400× N-acetylcysteine (81.5 mg/mL) | 1× (0.2 mg/mL) | 50 μL |
| Y27632 (10 mM)a | 10 μM | 20 μL |
| Total | n/a | 20 mL |
It can be stored at 4°C be used up to 10 days.
