REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
TeSR-E8	Stemcell Technologies	Cat#05990
STEMdiff Neural Crest Differentiation Kit	Stemcell Technologies	Cat#08610
DMEM/F-12 with 15 mM HEPES and sodium bicarbonate, without L-glutamine	Sigma-Aldrich	Cat#D6421
Gentle Cell Dissociation Reagent (GCDR)	Stemcell Technologies	Cat#07174
Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free	Corning	Cat#356231
Matrigel hESC-Qualified Matrix, LDEV-free	Corning	Cat#354277
DPBS without CaCl2, without MgCl2	Sigma-Aldrich	Cat#D8537
Y27632	Miltenyi Biotec	Cat#130-103-922
Advanced DMEM/F-12	Thermo Fisher/Gibco	Cat#12634010
GlutaMAX 100×	Thermo Fisher/Gibco	Cat# 35050061
HEPES (1 M)	Thermo Fisher/Gibco	Cat#15630080
Penicillin-Streptomycin (10,000 U/mL)	Thermo Fisher/Gibco	Cat#15140122
N-Acetylcysteine	Sigma-Aldrich	Cat#A9165-5G
Nicotinamide	Sigma-Aldrich	Cat#N0636-100G
Recombinant Murine Noggin	PeproTech	Cat#250-38
EGF Recombinant Mouse Protein	Thermo Fisher/Gibco	Cat#PMG8043
Recombinant Human FGF-10	PeproTech	Cat#100-26
Gastrin I (human)	Tocris	Cat#3006
A83-01	Tocris	Cat#2939
B27 supplement 50× (serum-free)	Thermo Fisher/Gibco	Cat#17504044
Minimum Essential Medium (MEM)	Thermo Fisher/Gibco	Cat#11095080
Buffer RLT	Qiagen	Cat# 79216
2-Mercaptoethanol	Sigma	Cat#63689
Trypan Blue Solution, 0.4%	Thermo Fisher/Gibco	Cat#15250061
Bovine Serum Albumin solution 30% in DPBS, sterile	Sigma-Aldrich	Cat#A9576-50ML
Gentamicin solution 50 mg/mL	Sigma-Aldrich	Cat#G1397
Experimental models: Cell lines
Mouse: C57BL/6	Charles River	n/a
Cell line: BIONi014-A hiPSC	EBiSC	Cat#66540265
HA-R-Spondin1-Fc 293 T	Trevigen	Cat#3710-001-01
Other
Class II Biological Safety Cabinet	Thermo Scientific	Cat#51023654
CO2 Incubator	n/a	n/a
Refrigerated Centrifuge	n/a	n/a
37°C Water Bath	n/a	n/a
Pipette Boy		n/a
Micro pipette set	Eppendorf Research	Cat#3120000909
Inverted microscope	n/a	n/a
Hemocytometer	Sigma	Cat#Z359629
Clean bench cabinet	n/a	n/a
Stereo microscope	n/a	n/a
Ultra fine micro vannas scissors (straight)	n/a	n/a
Ultra fine micro vannas scissors (curved)	n/a	n/a
Ultra fine micro forceps (straight)	n/a	n/a
Ultra fine micro forceps (curved)	n/a	n/a
15 mL Conical tube	Greiner bio-one	188261
50 mL Conical tube	Greiner bio-one	210270
6-well plate	Corning	353046
24-well plates	Corning	353047
35 mm petri dish	Corning	430165
100 mm dish	Corning	353003
Cell scraper	Sarstedt	83.1832
1.5 mL tubes	Eppendorf	0030 123.328
10 μL unfiltered sterile pipette tip	Starlab	S1110-3700
10 μL filtered sterile pipette tip	Starlab	S1120-3810-C
100 μL filtered sterile pipette tip	Starlab	S1120-1840-C
200 μL filtered sterile pipette tip	Starlab	S1120-8810-C
1000 μL filtered sterile pipette tip	Starlab	S1126-7810
5 mL Serological pipette	Greiner Bio-one	606180
10 mL Serological pipette	Greiner Bio-one	607160
25 mL Serological pipette	Greiner Bio-one	760180
50 mL Serological pipette	Greiner Bio-one	768180