Fig. 1.

Schematic representation of EV separation by differential centrifugation. The differential centrifugation technique separates EVs according to their size and density into medium EVs (white, >200 nm) and small EVs (green, <200 nm). Medium to large EVs are first pelleted by centrifugation at 10,000–20,000×g. The supernatant from this initial centrifugation step is then spun down at 100,000–200,000×g to obtain small EVs