Table 2.
The detail information of SSR primers used in the population genetic study
| Primers ID | motifs | Forward primers (5' to 3’) | Reverse primers (5' to 3’) | Target fragment length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
| VA 1 | (TTG)8 | GTTTGGGAAAGAAACGTCGTCA | ACCGAACACTTAGTGTGCAAGT | 143-156 | 56 |
| VA 4 | (TTA)8 | ACCGTACAATGTAAGGGTAAACGA | TCCACTTAGTCAATTAGCCACACA | 189-243 | 55 |
| VA 8 | (TGT)7 | GAAACCCAATGTTCTTGCGGAA | AATACCCTTGCCTTACGCGTAA | 264-273 | 56 |
| VA 9 | (TG)9 | CTTATGTAGCTGGCGTGTTTGT | TTGACCTTGGATTTGGGCCATA | 192-224 | 56 |
| VA 11 | (TG)7 | CTGATCTAATAACTTGGCGCGC | TGTCGGTCTGTTTGAGTGAACT | 261-281 | 56 |
| VA 14 | (TCT)7 | CAACACGGCGAAAACGATGATA | TACGCATCACACAAACCACAAC | 232-247 | 56 |
| VA 16 | (TCA)7 | CTGTACCCGAGGCTCTGAGT | AAGACAAGCAAGAAGTTGTCGC | 166-187 | 56 |
| VA 19 | (TC)7 | CTGACCCAAGTAATCCTCTCCG | TGGACGGTGATGTTTTGGATCT | 127-145 | 56 |
| VA 21 | (TAA)7 | GGAGCTAAAGCCACTCGTGTAA | CCATACGCCCCCACATTTTTAC | 242-257 | 56 |
| VA 23 | (TA)8 | TTTGGTTTGGTCCCCTTGTACA | TTCTGACCCACTTCAGGTTCTG | 182-196 | 56 |
| VA 25 | (TA)10 | TTCTGACTCCGATTTTGCTGGA | CAAGAGTTTGCTTGCCACTGC | 113-145 | 58 |
| VA 26 | (GTTA)7 | TAGAAGAGAAGCAGACAAGGGC | ACAACAAATTCTAGTACAGGTCTCA | 217-225 | 53 |
| VA 51 | (CA)7 | AAAGCTTTCCATTGTGTGTCGG | CGCGCAAGTTTGAACCAATCTA | 261-275 | 56 |
| VA 52 | (CA)10 | TGCGCTTAACGTATGTCTGAGT | ATAACAATGAGTCCCACGTGGT | 169-223 | 56 |
| VA 53 | (ATT)7 | GGCATTAATCTCTCAACTCTGCAC | CATCCATAACAAGCTGGTGAGC | 206-245 | 56 |
| VA 54 | (ATG)8 | TGGGACATCAATGGGATAAGAGT | CCCTTCCTCTTCAGCCTCATTT | 162-186 | 57 |
| VA 55 | (ATG)7 | TGGGACAAAACTAGAGGCTGTC | CCAGCTTCCGGTTGTATGTTTG | 171-183 | 57 |
| VA 56 | (ATC)9 | GTAGCTTGTGTGTCAGATCGGA | CACATCACCCATTGCAAGTGAG | 244-259 | 57 |
| VA 69 | (AG)7 | GTTCACGAATTGAAGCGGTTGA | GCGTTTGGCTCATCTTCAACAT | 110-136 | 56 |
| VA 70 | (AG)10 | ATTTCCAGGTTCGAAGCTCCTT | CACTCACATTTTAGTCAGTTGCGA | 117-147 | 55 |
| VA 80 | (ACTT)6 | GTCCTCCCATGACCACATTCTT | AGTACTCTCTGAGCGGTGTTTG | 186-206 | 57 |