Fig. 1.

Protein expression in neuronic stem cells from WFS patients and controls before and after tunicamycin administration measured by proteomics experiment. A Log₁₀ mean abundance of Wolframin across study groups. Whiskers represent standard deviation. One-way ANOVA p < 0.0001, p values of post-hoc Tukey tests are shown above corresponding groups; B Principle Component Analysis plot with annotated study groups. Arrows were added to components 1 and 2 as explanatory due to the observed effects of experimental reactions; C Heatmap with hierarchical clusterization performed on 50 proteins with the highest variance. Color scale represents row-standardized protein levels; D Volcano plot showing differential expression between WFS and healthy before tunicamycin administration. Significantly (FDR < 0.05) up-regulated (FC > 2) proteins are depicted in red and down-regulated (FDR < 0.05 and FC < 0.5) proteins are depicted in blue